Objectives.

Sepsis is a syndrome that results from a dysregulated host response to infection which can rapidly lead to organ dysfunction and death.
The aim of this study was the microbiological and clinical assessment of a new real-time PCR test (SeptiFast: SF, Roche Diagnostics) for the diagnosis of blood stream infections (BSI) in a population of neutropenic patients.
The results of SF were compared with blood cultures (BC) obtained on the same specimens and the clinical significance of the two methods was assessed.

Methods.

This mono-center study was conducted from the beginning of May 2006 until the end of July 2006. Eighty-six samples were obtained simultaneously for culture and SF from peripheral blood of 51 onco-hematologic patients with clinical suspicion of  bacterial or fungal BSI. All patients included in the study exhibited leukopenia (WBC < 200/ml).  The SF was performed following manufacturer’s instructions. BC was performed by using an automated system (BacTAlert, BioMerieux).  Standard laboratory procedures were employed for identification and antimicrobial susceptibility testing of the bacterial isolates from BC.

Results.

I am a biomedical engineering laboratory, I am specializing in molecular and cellular biology.

Now I'm determinated to open this blog during my Experimental thesis to compare with the scientific community.

Not leass important aim is to have suggestions on the design and discussion of experiments on the entire replication of the Human Cytomegalovirus

This blog will be a laboratory notebook open to the scientific community. It will be active soon.